Preparation of activated supports containing low pK amino groups. A new tool for protein immobilization via the carboxyl coupling method

作者: R. Fernandez-Lafuente , C.M. Rosell , V. Rodriguez , C. Santana , G. Soler

DOI: 10.1016/0141-0229(93)90016-U

关键词:

摘要: … of new amine-agarose gels containing primary amino groups with a very low pK value. These groups exhibit monoaminoethyl-N-aminoethyl structures (MANAagarose gels, Spanish …

参考文章(4)
R.G. Harris, J.J.M. Rowe, P.S. Stewart, D.C. Williams, Affinity chromatography of β-glucuronidase FEBS Letters. ,vol. 29, pp. 189- 192 ,(1973) , 10.1016/0014-5793(73)80558-7
K.L. Carraway, D.E. Koshland, [56] Carbodiimide modification of proteins Methods in Enzymology. ,vol. 25, pp. 616- 623 ,(1972) , 10.1016/S0076-6879(72)25060-1
JoséM Guisán, None, Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes Enzyme and Microbial Technology. ,vol. 10, pp. 375- 382 ,(1988) , 10.1016/0141-0229(88)90018-X
D.N. Salter, J.E. Ford, K.J. Scott, P. Andrews, Isolation of the folate-binding protein from cow's milk by the use of affinity chromatography FEBS Letters. ,vol. 20, pp. 302- 306 ,(1972) , 10.1016/0014-5793(72)80092-9