作者: B. Gregory Louis , P. S. Fitt
DOI: 10.1042/BJ1270069
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摘要: 1. The subunits α and β of Halobacterium cutirubrum DNA-dependent RNA polymerase have been purified to electrophoretic homogeneity. Both mol.wt. 18000 they are required in equimolar amounts for optimum activity. 2. instability the complete enzyme, αβ, absence salt is due rapid inactivation subunit these conditions. 3. Nearest-neighbour analysis product formed on poly[d(A-T)] as template shows that enzyme copies latter accurately. 4. initiates new chains with purine nucleoside triphosphates exclusively. 5. obtained standard assay conditions contains some high (>16S) material, but consists primarily short chains, average length 70–80 nucleotide units. 6. specificity has studied at low ionic strength. Its extreme dependence concentration unrelated gross overall base composition DNA used. 7. T7 transcribed asymmetrically selectively `early' genes. 8. Preliminary amino acid analyses show their content acidic, basic neutral acids does not differ appreciably from Escherichia coli polymerase.