Trans-differentiation of human mesenchymal stem cells generates functional hepatospheres on poly(L-lactic acid)-co-poly(ε-caprolactone)/collagen nanofibrous scaffolds

摘要: Mesenchymal stem cell (MSC)-based liver tissue engineering on nanofibrous scaffold holds great promise for cell-based therapy in injuries and end-stage failure treatments. We investigated the hepatic trans-differentiation potential of human MSCs a biocomposite poly(L-lactic acid)-co-poly (e-caprolactone)/collagen (PLACL/collagen) scaffold. The scaffolds comprised PLACL, collagen PLACL/collagen blend (2 : 1) were fabricated by electrospinning also evaluated fiber morphology, surface wettability, functional groups, porosity tensile properties. Hepatic bone marrow-derived (hMSCs) was carried out these over period 28 days using sequential induction with hepatogenic growth factors. Hepatogenesis confirmed scanning electron microscopy (SEM), phenotype tracking dye expression, quantitative expression genes, immunofluorescence staining hepatocyte-specific markers albumin release. results proved that porous supported enhanced hMSC proliferation compared to individual PLACL as well monolayer culture plate (TCP). Interestingly, hMSC-derived hepatocyte-like cells could aggregate form ‘hepatospheres’ similar normal spheroids. present study concludes are potentially biomimetic upon factors/cytokines, it augments hMSCs towards hepatosphere formation. Such bioengineered construct provides promising approach cellular damaged livers