Improved colorimetric assay for detecting influenza B virus neutralizing antibody responses to vaccination and infection.

作者: G A Tannock , J A Paul , R Herd , R D Barry , A L Reid

DOI: 10.1128/JCM.27.3.524-528.1989

关键词:

摘要: An automated neutralization test for influenza B virus is described in which antibody titers are determined according to the release of neutral red from infected or uninfected cells Madin-Darby canine kidney line. Endpoints a standard enzyme-linked immunosorbent assay reader. The requires no expensive immunologic reagents and was used evaluate responses both vaccination natural infection against virus. Overall were comparable with those obtained by hemagglutination inhibition, using Tween-ether-split B/Ann Arbor/1/86 as antigen (the HI-TE test). sensitivities compared two vaccines 88 89%; specificities lower at 61 60%, respectively. Responses vaccination, measured significantly higher split whole However, seroconversion tests observed 5 10 individuals whom detected either culture nasal throat washings presence immunofluorescence washings.

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