Quantitative-Qualitative Data Acquisition Using a Benchtop Orbitrap Mass Spectrometer

摘要: Current approaches to discovery-stage drug metabolism studies (pharmacokinetics, microsomal stability, etc. ) typically use triple-quadrupole-based for quantitative analysis. This necessitates the optimization of parameters such as Q1 and Q3 m/z values, collision energy, interface voltages. These detect only specified compound information about other components, metabolites, is lost. The ability perform full-scan acquisition analysis would eliminate need while enabling detection metabolites non-drug-related endogenous components. Such an instrument have provide sensitivity, selectivity, dynamic range, scan speed suitable studies. In this study, a prototype benchtop Orbitrap-based mass analyzer was used collect both qualitative data from human incubation samples well rat plasma pharmacokinetic Instrumental speed, resolution, accuracy are discussed in relation requirements quantitative-qualitative workflow. highly selective simultaneously characterizing vitro vivo discussed.