Chapter 3 Theory and Practice of High-Speed Microbore HPLC

摘要: Publisher Summary This chapter describes the theory and practice of high-speed microbore high performance liquid chromatography (HPLC). The also explores absolute limits to separation speed as predicted by well-established theory. development HPLC systems has received less attention than gas chromatography. They were able achieve complete resolution seven aromatic compounds on packed columns (1 m I.D.) within 20 30 seconds. continuing interest in (6–10) its application separations gives impression that is intrinsically faster larger bore columns. It can become more obvious from equations presented size little effect upon speed. Some advantages narrow-bore are becoming evident, such reductions wall effects or improved dissipation frictional heat. In addition, because be efficiently coupled, shorter column segments connected produce lengths closely matched needs. Microbore attractive for a variety practical, rather theoretical reasons.