FTIR-ATR detection of proteins and small molecules through DNA conjugation
作者： Wei Liao , Fang Wei , Dan Liu , Min Xie Qian , Gu Yuan
DOI: 10.1016/J.SNB.2005.06.021
关键词:
摘要: With the help of chemical modification silicon surfaces, FTIR-ATR was developed into a biosensing method to monitor biochemical processes on surfaces. In this proof-of principle work, crystals were covalently modified by oligonucleotides and two model systems investigated, including recognition anti-thrombin aptamer with thrombin organic molecule binding double helix DNA sequence-specific photocleavage. The affinity photocleavage reagent towards quantitatively measured. Thus, it shown that proposed FTIR-ATR-based allows for high-sensitive label-free detection various biological systems.
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