The retrograde transport of horseradish peroxidase from the developing limb of the chick embryo

摘要: Chick embryos ranging in age from 4.0 to 18 days of incubation and 1-2-day-old hatchlings received injections HRP solutions directly into the leg musculature. After survival periods 5 25 h motoneurons cells spinal sensory ganglia were found be stained with reaction product. It was that first appearance a positive coincided time when nerve processes are detected limb-bud by silver techniques (i.e. at 4.5 incubation). Only neurons region injection showed reaction. The demonstration retrograde transport mechanism axons which still undergoing growth differentiation provides possible for peripheral regulation certain features CNS neurogenesis. application this technique during development may also allow one map neuroanatomical pathways their formation.