NEURORECEPTOR mRNA EXPRESSION IN THE RAT MESENTERIC ARTERY DEVELOPS INDEPENDENTLY OF INNERVATION

摘要: Abstract Studies in autonomic targets have shown that nerves may be required for the development and maintenance of postsynaptic receptor populations. We examined this relationship rat mesenteric artery, assessing mRNA expression levels a range neuroreceptors after neonatal sympathectomy, using 6-hydroxydopamine or antisera directed against nerve growth factor, sensory denervation, capsaicin. Total RNA was extracted from 28 day old rats reverse transcription-polymerase chain reaction performed, primers specific α 1(A,B,D) - 2(A,B,C) -adrenergic, neurokinin (NK1–NK3), muscarinic (M1–M5) P 2X purinergic (P 2X1–7 ) families. Results showed no decreases any subtypes either sympathetic denervation. Small increases were detected following denervation some subtypes. conclude neither nor are mandatory vascular bed rat.