Gene manipulation and expression using genomic elements

摘要: Expression of mammalian target genes is achieved by employing chromosomal DNA, either native primary cells or YACs in a yeast host, where the include fragment chromosome, comprising gene. Employing homologous recombination, an amplifiable gene integrated into at site to allow for amplification. In same step, one more steps, as desired, and/or transcriptional system may be modified vivo mutagenesis. The resulting construct from recombination then transformed expression host and genome, randomly recombination. amplified appropriate agent providing multiple copies grown provide high yields desired wild-type protein.