In vitro pre-vascularisation of tissue-engineered constructs A co-culture perspective

摘要: In vitro pre-vascularization is one of the main vascularization strategies in tissue engineering field. Culturing cells within a tissue-engineered construct (TEC) prior to implantation provides researchers with greater degree control over fate cells. However, balancing diverse range different cell culture parameters seldom easy and most cases, especially highly vascularized tissues, more than type will reside system. multiple types same presents its own unique challenges pitfalls. The following review examines endothelial-driven evaluates direct indirect role other have vessel capillary formation. article then analyses can modulate co-culture system order design optimal constructs match desired clinical applications.