A novel derivatization of phenol after extraction from human serum using perfluorooctanoyl chloride for gas chromatography-mass spectrometric confirmation and quantification

摘要: Phenol (carbolic acid) is widely used as a disinfectant well in the chemical industry an intermediate synthesis of variety chemicals. also major metabolite benzene which many commercial solvents. toxic and caustic may cause death even from dermal absorption. Therefore, measurement phenol postmortem blood essential. The concentration can be measured by gas chromatography with flame ionization or mass spectrometry. analyzed high performance liquid chromatography. However, forensic toxicology, unambiguous confirmation spectrometry important quantification blood. Here we describe novel derivatization after extraction chloroform human serum using perfluorooctanoyl chloride. derivative showed strong molecular ion at m/z 490 (relative abundance: 23%) whereas base peak was observed 77. internal standard 3,4-dimethylphenol very 518 56%) 121. p-cresol, chemically related phenolic compound, 504 54%) 107. We baseline separation between derivatized (retention time: 6.1 min), p-cresol 7.8 9.4 min). no interferences our assay grossly hemolyzed serum. Within run precision studied containing 25 mg/L phenol. within 6.6% (mean = 24.3, SD 1.6 mg/L, n 8) 8.6% 25.5, 2.2 8). linear for concentrations 10-200 mg/L. detection limit 1 concentration. average recoveries were 92.1% to 94.0% various concentrations.