作者: Michael H.L. Green , Jillian E. Lowe , Carol A. Delaney , Irene C. Green
DOI: 10.1016/S0076-6879(96)69026-0
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摘要: Publisher Summary This chapter discusses the use of comet assay to detect nitric oxide-dependent DNA damage in mammalian cells. After hydrogen peroxide, oxide (NO) is most prevalent mutagen which human exposed. NO may interact with cellular amines form N-nitroso compounds. Its oxidation products nitrite and higher nitrogen oxides also have biological activity. produce strand breakage cells by precipitation assay, situ nick translation, assay. The a sensitive method for detection breaks cell. detects release from highly supercoiled DNA-protein complex. In comparison other methods, relatively robust economical its material. It has specific advantage that as single-cell it can nonuniform response within cell population, characterize behavior different types mixed population. Only small proportion DNA-damaging agents, including ionizing radiation, bleomycin, induces direct phosphodiester backbone.