Comet assay to detect nitric oxide-dependent DNA damage in mammalian cells.

作者: Michael H.L. Green , Jillian E. Lowe , Carol A. Delaney , Irene C. Green

DOI: 10.1016/S0076-6879(96)69026-0

关键词:

摘要: Publisher Summary This chapter discusses the use of comet assay to detect nitric oxide-dependent DNA damage in mammalian cells. After hydrogen peroxide, oxide (NO) is most prevalent mutagen which human exposed. NO may interact with cellular amines form N-nitroso compounds. Its oxidation products nitrite and higher nitrogen oxides also have biological activity. produce strand breakage cells by precipitation assay, situ nick translation, assay. The a sensitive method for detection breaks cell. detects release from highly supercoiled DNA-protein complex. In comparison other methods, relatively robust economical its material. It has specific advantage that as single-cell it can nonuniform response within cell population, characterize behavior different types mixed population. Only small proportion DNA-damaging agents, including ionizing radiation, bleomycin, induces direct phosphodiester backbone.

参考文章(42)
Narendra P Singh, Michael T McCoy, Raymond R Tice, Edward L Schneider, None, A simple technique for quantitation of low levels of DNA damage in individual cells Experimental Cell Research. ,vol. 175, pp. 184- 191 ,(1988) , 10.1016/0014-4827(88)90265-0
H. Ohshima, H. Bartsch, Chronic infections and inflammatory processes as cancer risk factors: possible role of nitric oxide in carcinogenesis Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. ,vol. 305, pp. 253- 264 ,(1994) , 10.1016/0027-5107(94)90245-3
Katarina Victorin, Review of the genotoxicity of nitrogen oxides Mutation Research\/reviews in Genetic Toxicology. ,vol. 317, pp. 43- 55 ,(1994) , 10.1016/0165-1110(94)90011-6
Osamu Nikaido, Susan A. Harcourt, Alastair P. W. Waugh, Jane Cole, Toshio Mori, Michael H. L. Green, Colin F. Arlett, Jillian E. Lowe, Brian L. Diffey, Leendert Roza, Hypersensitivity of Human Lymphocytes to UV-B and Solar Irradiation Cancer Research. ,vol. 53, pp. 609- 614 ,(1993)
I.A. Brazell, P.R. Cook, Supercoils in human DNA Journal of Cell Science. ,vol. 19, pp. 261- 279 ,(1975) , 10.1242/JCS.19.2.261
K. Fehsel, A. Jalowy, S. Qi, V. Burkart, B. Hartmann, H. Kolb, Islet Cell DNA Is a Target of Inflammatory Attack by Nitric Oxide Diabetes. ,vol. 42, pp. 496- 500 ,(1993) , 10.2337/DIAB.42.3.496
Andreas Hartmann, Ulla Plappert, Klaus Raddata, Martina Grünert-Fuchs, Günter Speit, Does physical activity induce DNA damage Mutagenesis. ,vol. 9, pp. 269- 272 ,(1994) , 10.1093/MUTAGE/9.3.269
Beatrice L. Pool‐Zobel, Barbara Bertram, Martin Knoll, Renate Lambertz, Christian Neudecker, Ulrich Schillinger, Peter Schmezer, Wilhelm H. Holzapfel, Antigenotoxic properties of lactic acid bacteria in vivo in the gastrointestinal tract of rats. Nutrition and Cancer. ,vol. 20, pp. 271- 281 ,(1993) , 10.1080/01635589309514295
Akiko Uzawa, Gen Suzuki, Yukiko Nakata, Makoto Akashi, Harumi Ohyama, Atsuo Akanuma, Radiosensitivity of CD45RO+ memory and CD45RO- naive T cells in culture. Radiation Research. ,vol. 137, pp. 25- 33 ,(1994) , 10.2307/3578787