Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase.
作者： KunLiang Guan , Jack E. Dixon
DOI: 10.1016/0003-2697(91)90534-Z
关键词:
摘要: … fusion protein with free glutathione, the washed agarose beads, to which the fusion protein is … The vector pGEX-2T has been used to express the protein tyrosine phosphatase (PTPU323…
elsevier.com
sci-hub.se 参考文章(10)
B. Nilsson, L. Abrahmsén, M. Uhlén, Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors The EMBO Journal. ,vol. 4, pp. 1075- 1080 ,(1985) , 10.1002/J.1460-2075.1985.TB03741.X
Z Zhu, O M Andrisani, D A Pot, J E Dixon, Purification and characterization of a 43-kDa transcription factor required for rat somatostatin gene expression. Journal of Biological Chemistry. ,vol. 264, pp. 6550- 6556 ,(1989) , 10.1016/S0021-9258(18)83383-X
F A Marston, The purification of eukaryotic polypeptides synthesized in Escherichia coli. Biochemical Journal. ,vol. 240, pp. 1- 12 ,(1986) , 10.1042/BJ2400001
Helmut M. Sassenfeld, Stephen J. Brewer, A Polypeptide Fusion Designed for the Purification of Recombinant Proteins Nature Biotechnology. ,vol. 2, pp. 76- 81 ,(1984) , 10.1038/NBT0184-76
Peter C. Simons, David L. Vander Jagt, Purification of glutathione S-transferases from human liver by glutathione-affinity chromatography. Analytical Biochemistry. ,vol. 82, pp. 334- 341 ,(1977) , 10.1016/0003-2697(77)90169-5
Jui-Yoa CHANG, Thrombin specificity. Requirement for apolar amino acids adjacent to the thrombin cleavage site of polypeptide substrate. FEBS Journal. ,vol. 151, pp. 217- 224 ,(1985) , 10.1111/J.1432-1033.1985.TB09091.X
David P. Gearing, Nicos A. Nicola, Donald Metcalf, Simon Foote, Tracy A. Willson, Nicholas M. Gough, R. Lindsay Williams, Production of Leukemia Inhibitory Factor in Escherichia coli by a Novel Procedure and Its Use in Maintaining Embryonic Stem Cells in Culture Nature Biotechnology. ,vol. 7, pp. 1157- 1161 ,(1989) , 10.1038/NBT1189-1157
Donald B. Smith, Kevin S. Johnson, Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase Gene. ,vol. 67, pp. 31- 40 ,(1988) , 10.1016/0378-1119(88)90005-4
U. K. LAEMMLI, Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 Nature. ,vol. 227, pp. 680- 685 ,(1970) , 10.1038/227680A0
B. Löwenadler, B. Nilsson, L. Abrahmsén, T. Moks, L. Ljungqvist, E. Holmgren, S. Paleus, S. Josephson, L. Philipson, M. Uhlén, Production of specific antibodies against protein A fusion proteins. The EMBO Journal. ,vol. 5, pp. 2393- 2398 ,(1986) , 10.1002/J.1460-2075.1986.TB04509.X