Disruption of the gene C12orf35 leads to increased productivities in recombinant CHO cell lines.
作者： Anett Ritter , Tatjana Rauschert , Mevion Oertli , Daniel Piehlmaier , Panagiotis Mantas
DOI: 10.1002/BIT.26009
关键词:
摘要: Recently, we reported that the loss of a telomeric region chromosome 8 in Chinese Hamster Ovary (CHO) cells correlates with higher recombinant productivities. New cell lines lacking this region, called CHO-C8DEL, showed several advantages during line generation and for production proteins (Ritter et al., 2016, Biotechnol Bioeng). Here, performed knock-down knock-out experiments genes located within to identify causing observed phenotypes CHO-C8DEL lines. We present evidence or reduced expression gene C12orf35 is responsible productivities shorter recovery times selection pressure. These effects are mediated by increased levels mRNA exogenes heavy chain (HC) light (LC) as well dihydrofolate reductase (DHFR) neomycin phosphotransferase (Neo) stable antibodies. Biotechnol. Bioeng. 2016;113: 2433-2442. © 2016 Wiley Periodicals, Inc.
sci-hub.se 参考文章(54)
Mariati, Esther YC Koh, Jessna HM Yeo, Steven CL Ho, Yuansheng Yang, Toward stable gene expression in CHO cells Bioengineered bugs. ,vol. 5, pp. 340- 345 ,(2014) , 10.4161/BIOE.32111
Jahir M. Gutierrez, Nathan E. Lewis, Optimizing eukaryotic cell hosts for protein production through systems biotechnology and genome-scale modeling. Biotechnology Journal. ,vol. 10, pp. 939- 949 ,(2015) , 10.1002/BIOT.201400647
Simon Fischer, René Handrick, Kerstin Otte, The art of CHO cell engineering: A comprehensive retrospect and future perspectives Biotechnology Advances. ,vol. 33, pp. 1878- 1896 ,(2015) , 10.1016/J.BIOTECHADV.2015.10.015
Anett Ritter, Bernd Voedisch, Johannes Wienberg, Burkhard Wilms, Sabine Geisse, Thomas Jostock, Holger Laux, Deletion of a telomeric region on chromosome 8 correlates with higher productivity and stability of CHO cell lines Biotechnology and Bioengineering. ,vol. 113, pp. 1084- 1093 ,(2016) , 10.1002/BIT.25876
William C. Yang, Jiuyi Lu, Ngan B. Nguyen, An Zhang, Nicholas V. Healy, Rashmi Kshirsagar, Thomas Ryll, Yao-Ming Huang, Addition of valproic acid to CHO cell fed-batch cultures improves monoclonal antibody titers. Molecular Biotechnology. ,vol. 56, pp. 421- 428 ,(2014) , 10.1007/S12033-013-9725-X
Wei Zheng, Hongyu Zhao, Eugenio Mancera, Lars M. Steinmetz, Michael Snyder, Genetic analysis of variation in transcription factor binding in yeast Nature. ,vol. 464, pp. 1187- 1191 ,(2010) , 10.1038/NATURE08934
Jędrzej Małecki, Angela Y. Y. Ho, Anders Moen, Helge-André Dahl, Pål Ø. Falnes, Human METTL20 is a mitochondrial lysine methyltransferase that targets the β subunit of electron transfer flavoprotein (ETFβ) and modulates its activity. Journal of Biological Chemistry. ,vol. 290, pp. 423- 434 ,(2015) , 10.1074/JBC.M114.614115
Peter M. O'Callaghan, Jane McLeod, Leon P. Pybus, Clare S. Lovelady, Stephen J. Wilkinson, Andrew J. Racher, Alison Porter, David C. James, Cell line‐specific control of recombinant monoclonal antibody production by CHO cells Biotechnology and Bioengineering. ,vol. 106, pp. 938- 951 ,(2010) , 10.1002/BIT.22769
Haimanti Dorai, Blanca Csirke, Bernie Scallon, Subinay Ganguly, Correlation of heavy and light chain mRNA copy numbers to antibody productivity in mouse myeloma production cell lines. Hybridoma. ,vol. 25, pp. 1- 9 ,(2006) , 10.1089/HYB.2006.25.1
Matthew P. Zustiak, Lisa Jose, Yueqing Xie, Jianwei Zhu, Michael J. Betenbaugh, Enhanced transient recombinant protein production in CHO cells through the co-transfection of the product gene with Bcl-xL. Biotechnology Journal. ,vol. 9, pp. 1164- 1174 ,(2014) , 10.1002/BIOT.201300468