Enhanced gastric cancer growth potential of mesenchymal stem cells derived from gastric cancer tissues educated by CD4+ T cells.

摘要: Objectives Gastric cancer mesenchymal stem cells (GC-MSCs) can promote the development of tumour growth. The tumour-promoting role tumour-associated MSCs and T has been demonstrated. as major immune may influence induce a pro-tumour phenotype in MSCs. This study focused on whether CD4+ affect GC-MSCs to gastric growth. Materials methods CD4+ upregulation programmed death ligand 1 (PD-L1) expression through phosphorylated signal transducer activator transcription (p-STAT3) signalling pathway was confirmed by immunofluorescence, western blotting RT-PCR. Migration GC detected Transwell migration assay, apoptosis measured flow cytometry using annexin V/propidium iodide double staining. cell-primed promoted growth subcutaneously transplanted model BALB/c nu/nu mice. Results Gastric stimulated activated enhanced potential xenografts. PD-L1 mediated p-STAT3 pathway. cells-primed have greater volume rate-promoting than GC-MSCs, with cell-intrinsic PD-1/mammalian target rapamycin (mTOR) activation. Conclusions This showed that are plastic. immunophenotype changes cell research based interaction between cells, providing new understanding immunotherapy GC.