Studies on a nuclease from Ustilago maydis. I. Purification, properties, and implication in recombination of the enzyme.
作者： William K. Holloman , Robin Holliday
DOI: 10.1016/S0021-9258(19)43199-2
关键词:
摘要: Abstract A nuclease highly active on denatured DNA or RNA has been purified 8,600-fold from the smut fungus Ustilago maydis. The molecular weight of enzyme is 42,000 as determined by gel filtration. Added divalent cations are not required for activity. Ethylenediaminetetraacetate strongly inhibits activity, but simultaneous addition an excess Zn2+ Co2+ reverses inhibition. Other inhibitors include potassium phosphate, 2-mercaptoethanol, and ATP. a low level activity native DNA. Certain mutant strains which unable to carry out allelic recombination have reduced amounts enzyme. Therefore, this may play some role in organism.
sci-hub.st 参考文章(52)
Stuart Linn, I.R. Lehman, An Endonuclease from Neurospora crassa Specific for Polynucleotides Lacking an Ordered Structure Journal of Biological Chemistry. ,vol. 240, pp. 1287- 1293 ,(1965) , 10.1016/S0021-9258(18)97573-3
Dean Fraser, Elizabeth A. Jerrel, The amino acid composition of T3 bacteriophage. Journal of Biological Chemistry. ,vol. 205, pp. 291- 295 ,(1953) , 10.1016/S0021-9258(19)77254-8
Stuart Linn, I.R. Lehman, An Endonuclease from Neurospora crassa Specific for Polynucleotides Lacking an Ordered Structure Journal of Biological Chemistry. ,vol. 240, pp. 1294- 1304 ,(1965) , 10.1016/S0021-9258(18)97574-5
Charles M. Radding, John Josse, Arthur Kornberg, Enzymatic synthesis of deoxyribonucleic acid. XII. A polymer of deoxyguanylate and deoxycytidylate. Journal of Biological Chemistry. ,vol. 237, pp. 2869- 2876 ,(1962) , 10.1016/S0021-9258(18)60243-1
E. Signer, H. Echols, J. Weil, C. Radding, M. Shulman, L. Moore, K. Manly, The general recombination system of bacteriophage lambda. Cold Spring Harbor Symposia on Quantitative Biology. ,vol. 33, pp. 711- 714 ,(1968) , 10.1101/SQB.1968.033.01.080
I.R. Lehman, G.G. Roussos, E.A. Pratt, The deoxyribonucleases of Escherichia coli. II. Purification and properties of a ribonucleic acid-inhibitable endonuclease. Journal of Biological Chemistry. ,vol. 237, pp. 819- 828 ,(1962) , 10.1016/S0021-9258(18)60378-3
Stuart Linn, [31] An endonuclease from Neurospora crassa specific for polynucleotides lacking an ordered structure Methods in Enzymology. ,vol. 12, pp. 247- 255 ,(1967) , 10.1016/S0076-6879(67)12038-7
Gerald F. Vovis, Gérard Buttin, An ATP-dependent deoxyribonuclease from Diplococcus pneumoniae. II. Evidence for its involvement in bacterial recombination. Biochimica et Biophysica Acta. ,vol. 224, pp. 42- 54 ,(1970) , 10.1016/0005-2787(70)90618-0
Eileen A. Friedman, Hamilton O. Smith, An Adenosine Triphosphate-dependent Deoxyribonuclease from Hemophilus influenzae Rd Journal of Biological Chemistry. ,vol. 247, pp. 2846- 2853 ,(1972) , 10.1016/S0021-9258(19)45288-5
Motoaki Anai, Takakata Hirahashi, Yasuyuki Takagi, A Deoxyribonuclease Which Requires Nucleoside Triphosphate from Micrococcus lysodeikticus I. PURIFICATION AND CHARACTERIZATION OF THE DEOXYRIBONUCLEASE ACTIVITY Journal of Biological Chemistry. ,vol. 245, pp. 767- 774 ,(1970) , 10.1016/S0021-9258(18)63330-7