Spatial Distribution of Glycerophospholipids in the Ocular Lens

摘要: Knowledge of the spatial distribution lipids in intraocular lens is important for understanding physiology and biochemistry this unique tissue gaining a better insight into mechanisms underlying diseases lens. Following our previous study showing sphingolipids porcine lens, current used ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOFMS) to provide whole lipidome these studies were supplemented by matrix-assisted laser desorption ionization imaging (MALDI MSI) using ultra-high resolution Fourier transform ion cyclotron resonance (FTICR MS) determine glycerophospholipids. Altogether 172 lipid species identified with high confidence their concentration was determined. Sphingomyelins, phosphatidylcholines, phosphatidylethanolamines most abundant classes. We then determined concentration-dependent distributions 20 6 phosphatidylethanolamines, 4 phosphatidic acids. Based on planar molecular images lipids, we report organization fiber cell membranes within ocular suggest roles normal diseased lenses.