Infection of capilloviruses requires subgenomic RNAs whose transcription is controlled by promoter-like sequences conserved among flexiviruses

作者: Ken Komatsu , Hisae Hirata , Takako Fukagawa , Yasuyuki Yamaji , Yukari Okano

DOI: 10.1016/J.VIRUSRES.2012.02.019

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摘要: The first open-reading frame (ORF) of apple stem grooving virus (ASGV), of the genus Capillovirus, encodes an apparently chimeric polyprotein containing conserved regions for replicase (Rep) and coat protein (CP). However, our previous study revealed that ASGV mutants with distinct and discontinuous Rep-and CP-coding regions successfully infect plants, indicating that CP expressed via a subgenomic RNA (sgRNA) is sufficient for viability of the virus. Here we identified a transcription start site of the CP sgRNA and revealed that …

参考文章(19)
G. P. Martelli, T. Candresse, S. Namba, Trichovirus, a new genus of plant viruses. Archives of Virology. ,vol. 134, pp. 451- 455 ,(1994) , 10.1007/BF01310583
Águeda Renovell, Selma Gago, Susana Ruiz-Ruiz, Karelia Velázquez, Luis Navarro, Pedro Moreno, Mari Carmen Vives, José Guerri, Mapping the subgenomic RNA promoter of the Citrus leaf blotch virus coat protein gene by Agrobacterium-mediated inoculation Virology. ,vol. 406, pp. 360- 369 ,(2010) , 10.1016/J.VIROL.2010.07.034
Motoyasu OCHI, Satoshi KASHIWAZAKI, Kazuyuki HIRATSUKA, Shigetou NAMBA, Tsuneo TSUCHIZAKI, Nucleotide Sequence of the 3'-Terminal Region of Potato Virus T RNA Japanese Journal of Phytopathology. ,vol. 58, pp. 416- 425 ,(1992) , 10.3186/JJPHYTOPATH.58.416
M. Russo, L. Rubino, A. De Stradis, Giovanni P. Martelli, The complete nucleotide sequence of potato virus T Archives of Virology. ,vol. 154, pp. 321- 325 ,(2009) , 10.1007/S00705-008-0300-X
M. J. Adams, J. F. Antoniw, M. Bar-Joseph, A. A. Brunt, T. Candresse, G. D. Foster, G. P. Martelli, R. G. Milne, S. K. Zavriev, C. M. Fauquet, The new plant virus family Flexiviridae and assessment of molecular criteria for species demarcation Archives of Virology. ,vol. 149, pp. 1045- 1060 ,(2004) , 10.1007/S00705-004-0384-X
Giovanni P. Martelli, Michael J. Adams, Jan F. Kreuze, Valerian V. Dolja, Family Flexiviridae: a case study in virion and genome plasticity. Annual Review of Phytopathology. ,vol. 45, pp. 73- 100 ,(2007) , 10.1146/ANNUREV.PHYTO.45.062806.094401
Nurbol Galiakparov, Dariusz E Goszczynski, Xibing Che, Ozgur Batuman, Moshe Bar-Joseph, Munir Mawassi, Two classes of subgenomic RNA of grapevine virus A produced by internal controller elements. Virology. ,vol. 312, pp. 434- 448 ,(2003) , 10.1016/S0042-6822(03)00239-3
Marı́a C. Vives, Luis Galipienso, Luis Navarro, Pedro Moreno, José Guerri, Characterization of Two Kinds of Subgenomic RNAs Produced by Citrus Leaf Blotch Virus Virology. ,vol. 295, pp. 328- 336 ,(2002) , 10.1006/VIRO.2001.1349
Hisae Hirata, Yasuyuki Yamaji, Ken Komatsu, Satoshi Kagiwada, Kenro Oshima, Yukari Okano, Shuichiro Takahashi, Masashi Ugaki, Shigetou Namba, Pseudo-polyprotein translated from the full-length ORF1 of capillovirus is important for pathogenicity, but a truncated ORF1 protein without variable and CP regions is sufficient for replication Virus Research. ,vol. 152, pp. 1- 9 ,(2010) , 10.1016/J.VIRUSRES.2010.03.016