Genetic ablation in transgenic mice.

摘要: The study of mammalian development has very quickly moved from a largely descriptive endeavour to one in which precise mechanistic questions can now be formulated and answered. Undoubtedly, advances this area have been the result strong foundation experimental embryology, application molecular genetic techniques isolation analysis genes developmental interest, ability manipulate genetically embryo through transgenic mouse technology. Perhaps most dramatic illustration power these new technologies is potential generate mice either that carry mutations virtually any gene germ line targeting totipotent embryonic stem (ES) cells or lack specific cell types ablation technology reviewed here. Together, two approaches made it possible knock out type an intact animal thus offer almost unlimited possibilities for addressing concerning cellular biology development. As well, models various human diseases such as dwarfism, immunodeficiencies demyelination generated. It clear further refinements both are necessary before full approach will realized. Further conditional inducible strategies, coupled with more definition cis-acting sequences, responsible directing expression fully differentiated primitive cells, greatly broaden range addressed by approach.(ABSTRACT TRUNCATED AT 250 WORDS)