Curve fitting approach for measurement of cellular osmotic properties by the electrical sensing zone method. II. Membrane water permeability.
作者： Adam Z. Higgins , Jens O.M. Karlsson
DOI: 10.1016/J.CRYOBIOL.2009.10.002
关键词:
摘要: In a companion paper, we demonstrated that dynamic range limitations can confound measurement of the osmotically inactive volume using electrical sensing zone instruments (e.g., Coulter counters), and presented an improved parameter estimation method in which lognormal function was fit to cell distribution allow extrapolation beyond bounds data. Presently, have investigated effect on membrane water permeability (L(p)), adapted for L(p) from transient An alternative strategy (the limit adjustment method, measured isotonic is used generate model predictions curve fitting, are adjusted such not required) also developed. The performance these new algorithms compared conventional method. best-fit values vitro experiments with mouse insulinoma (MIN6) cells differed significantly different techniques (p<0.001). Using silico experiments, shown be most accurate (relative error 0.4+/-3.2%), whereas underestimated by 19+/-2% MIN6 cells. Parametric analysis revealed associated sensitive width distribution. Our initial implementation yielded significant errors, accuracy this algorithm after including normalization scheme.
elsevier.com
sci-hub.se 参考文章(20)
C. Fedorow, L. E. Mcgann, G. S. Korbutt, G. R. Rayat, R. V. Rajotte, J. R. T. Lakey, Osmotic and cryoprotectant permeation characteristics of islet cells isolated from the newborn pig pancreas. Cell Transplantation. ,vol. 10, pp. 651- 659 ,(2001) , 10.3727/000000001783986323
P. Mazur, S.P. Leibo, E.H.Y. Chu, A two-factor hypothesis of freezing injury Experimental Cell Research. ,vol. 71, pp. 345- 355 ,(1972) , 10.1016/0014-4827(72)90303-5
A.Z. Higgins, J.O.M. Karlsson, Curve fitting approach for measurement of cellular osmotic properties by the electrical sensing zone method. I. Osmotically inactive volume Cryobiology. ,vol. 57, pp. 223- 233 ,(2008) , 10.1016/J.CRYOBIOL.2008.09.001
D.Y. Gao, Q. Chang, C. Liu, K. Farris, K. Harvey, L.E. McGann, D. English, J. Jansen, J.K. Critser, Fundamental Cryobiology of Human Hematopoietic Progenitor Cells I: Osmotic Characteristics and Volume Distribution Cryobiology. ,vol. 36, pp. 40- 48 ,(1998) , 10.1006/CRYO.1997.2060
G. S. Rule, P. Law, J. Kruuv, J. R. Lepock, Water permeability of mammalian cells as a function of temperature in the presence of dimethylsulfoxide: Correlation with the state of the membrane lipids Journal of Cellular Physiology. ,vol. 103, pp. 407- 416 ,(1980) , 10.1002/JCP.1041030306
Y Agca, J Liu, S Mullen, J Johnson-Ward, K Gould, A Chan, J Critser, Chimpanzee (Pan troglodytes) spermatozoa osmotic tolerance and cryoprotectant permeability characteristics. Journal of Andrology. ,vol. 26, pp. 470- 477 ,(2005) , 10.2164/JANDROL.04169
David J. Sheskin, Handbook of Parametric and Nonparametric Statistical Procedures ,(2007)
H Hayashi, K Inoue, S Shinohara, Y.J Gu, H Setoyama, Y Kawakami, T Yamasaki, W.X Cui, N Kinoshita, M Imamura, H Iwata, Y Ikada, J Miyazaki, New Approach by Tissue Engineering for Extended Selective Transplantation With a Pancreatic B-Cell Line (MIN6) Annual Congress of the Japan Society for Transplantation. ,vol. 30, pp. 83- 85 ,(1998) , 10.1016/S0041-1345(97)01187-1
E.J. Woods, J. Liu, J.A. Gilmore, T.J. Reid, D.Y. Gao, J.K. Critser, Determination of human platelet membrane permeability coefficients using the Kedem-Katchalsky formalism: estimates from two- vs three-parameter fits. Cryobiology. ,vol. 38, pp. 200- 208 ,(1999) , 10.1006/CRYO.1998.2146
Jun Liu, Michael A.J. Zieger, Jonathan RT Lakey, Erik J. Woods, John K. Critser, The determination of membrane permeability coefficients of canine pancreatic islet cells and their application to islet cryopreservation. Cryobiology. ,vol. 35, pp. 1- 13 ,(1997) , 10.1006/CRYO.1997.2018