Allium hooshidaryae Mashayekhi, Zarre & R.M. Fritsch (Alliaceae); Chemical Compositions, Biological and Ethnomedicine uses.

摘要: Abstract Ethnopharmacological relevance Allium hooshidaryae Mashayekhi, Zarre & R.M. Fritsch sp. nova (sect. Compactoprason) is a wild plant in northwestern Iran. The traditionally used, besides as spice, also for its medicinal properties. Aim of the study Due to shortcoming evidence scientific research and importance this folk medicine, aims assess chemical compositions biological activities, which have no longer reported date. Materials Methods bulbs A. were collected from West Azerbaijan, essential oil was obtained by hydrodistillation using Clevenger-type apparatus according European pharmacopeia. hydromethanolic extract maceration method. volatile evaluated use combined gas chromatography/flame ionization detector (GC/FID) chromatography/mass spectrometry (GC/MS) techniques. Furthermore, different activities yielded vitro evaluated. antibacterial antifungal assessed disc diffusion assay, tube dilution minimal inhibitory concentration (MIC), bactericidal (MBC), fungicidal (MFC). cytotoxic assayed reduction 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) toward two human cancerous cell lines (MOLT-4 MCF-7). Antioxidant activity investigated 2,2-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging assay. Results GC/FID GC/MS analyses allowed detecting 62 components representing 91.87% total oil. compounds identified comparison relative retention indices (RRI), mass spectra with those NIST08/NIH Wiley (257 7L) libraries co-elution authentic samples where available. Surprisingly, most abundant compound menthol (19.0%) followed carvacrol (10.1%), menthone (6.4%), methyl (methylthiomethyl) disulfide (4.2%), dimethyl (3.8%), thymol (3.8%). Contrary other species enriched sulfur compounds, just three accounting 10.7% sulfur-sulfur bond containing (Dimethyl disulfide, Methyl (methylthio) Bis-methylthiomethyl disulfide). showed higher anti-radical (IC50 DPPH 9.81 μg/mL) (for MOLT-4 MCF-7, IC50s 76.3 128.6 μg/mL, respectively) rather than that 39.9 μg/mL; IC50 109.2 MCF-7 297.5 μg/mL). While, exhibited anti-Staphylococcus aurous anti-Escherichia coli approximately same Chloramphenicol (positive control). MIC values 31.25 62.5 μg/mL disk inhibition zone 23 21 mm, respectively. In addition, Candida albicans had moderate sensitivity (MFC Conclusions shows potency be used food protection addition further investigations. Associated antimicrobial abilities both products, compatible results observed traditional claim having being not These findings will facilitate development deep