Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana.

摘要: Genetic engineering of plants that results in successful establishment new biochemical or regulatory pathways requires stable introduction one more genes into the plant genome. It might also be necessary to down-regulate turn off expression endogenous order reduce activity competing pathways. An established way knockdown gene is expressing a hairpin-RNAi construct, eventually leading degradation specifically targeted mRNA. Knockdown multiple do not share homologous sequences still challenging and involves either sophisticated cloning strategies create vectors with different serial constructs transformation events often restricted by lack available markers. Synthetic RNAi fragments were assembled yeast carrying six seven non-family introduced pAGRIKOLA. Transformation Arabidopsis thaliana subsequent analysis proved efficient all target genes. We present simple cost-effective method simultaneously sequence homology. The presented can applied animal synthetic biology as well traditional genetic engineering.