High frequency of fusion transcripts involving TCF7L2 in colorectal cancer: novel fusion partner and splice variants.

摘要: VTI1A-TCF7L2 was reported as a recurrent fusion gene in colorectal cancer (CRC), found to be expressed three out of 97 primary cancers, and one cell line, NCI-H508, where genomic deletion joins the two genes [1]. To investigate this further, we analyzed high-throughput DNA RNA sequencing data from seven CRC lines, identified RP11-57H14.3 (ENSG00000225292) novel partner for TCF7L2. The discovered both genome transcriptome HCT116 line. By triplicate nested RT-PCR, tested transcript expression series 106 tissues, 21 14 normal colonic mucosa, 20 tissues miscellaneous anatomical sites. Altogether, 42% 45% samples TCF7L2-RP11-57H14.3 transcripts, respectively. transcripts were seen 29% mucosa samples, 25% 75% other organs, revealing that TCF7L2 are neither specific nor colon rectum. Seven different splice variants detected fusion, which novel. Four fusion. In conclusion, have including gene, RP11-57H14.3, demonstrated detectable levels large fraction well tissue types. We suggest observed high frequency transcription induced chimeras at low most samples. similar by rearrangements individual lines may yet oncogenic potential suggested original study Bass et al.