Transcriptional Regulation of the Human TLR9 Gene
作者： Fumihiko Takeshita , Koichi Suzuki , Shin Sasaki , Norihisa Ishii , Dennis M. Klinman
DOI: 10.4049/JIMMUNOL.173.4.2552
关键词:
摘要: To clarify the molecular basis of human TLR9 (hTLR9) gene expression, activity hTLR9 promoter was characterized using myeloma cell line RPMI 8226. Reporter analysis and EMSA demonstrated that transcription regulated via four cis-acting elements, cAMP response element, 5'-PU box, 3'-PU a C/EBP site, interacted with CREB1, Ets2, Elf1, Elk1, C/EBPalpha factors. Other members family, such as C/EBPbeta, C/EBPdelta, C/EBPepsilon, were also important for transcription. CpG DNA-mediated suppression led to decreased binding trans-acting factors their corresponding elements. It appeared mediated c-Jun NF-kappaB p65 cooperation among culminated in maximal gene. These findings will help elucidate mechanism regulation provide insight into process by which evolved mammalian immune system.
jimmunol.org参考文章(49)
Hiroaki Hemmi, Osamu Takeuchi, Taro Kawai, Tsuneyasu Kaisho, Shintaro Sato, Hideki Sanjo, Makoto Matsumoto, Katsuaki Hoshino, Hermann Wagner, Kiyoshi Takeda, Shizuo Akira, A Toll-like receptor recognizes bacterial DNA. Nature. ,vol. 408, pp. 740- 745 ,(2000) , 10.1038/35047123
Fumihiko Takeshita, Cynthia A. Leifer, Ihsan Gursel, Ken J. Ishii, Saoko Takeshita, Mayda Gursel, Dennis M. Klinman, Cutting Edge: Role of Toll-Like Receptor 9 in CpG DNA-Induced Activation of Human Cells The Journal of Immunology. ,vol. 167, pp. 3555- 3558 ,(2001) , 10.4049/JIMMUNOL.167.7.3555
Arnaud Zaldumbide, Françoise Carlotti, Philippe Pognonec, Kim E. Boulukos, The Role of the Ets2 Transcription Factor in the Proliferation, Maturation, and Survival of Mouse Thymocytes Journal of Immunology. ,vol. 169, pp. 4873- 4881 ,(2002) , 10.4049/JIMMUNOL.169.9.4873
Mary Hanlon, Linda M Bundy, Linda Sealy, C/EBPBeta and Elk-1 synergistically transactivate the c-fos serum response element BMC Cell Biology. ,vol. 1, pp. 2- 2 ,(2000) , 10.1186/1471-2121-1-2
Gerd A. Blobel, CREB-binding protein and p300: molecular integrators of hematopoietic transcription Blood. ,vol. 95, pp. 745- 755 ,(2000) , 10.1182/BLOOD.V95.3.745.003K05_745_755
Viola Haehnel, Lucia Schwarzfischer, Matthew J. Fenton, Michael Rehli, Transcriptional Regulation of the Human Toll-Like Receptor 2 Gene in Monocytes and Macrophages Journal of Immunology. ,vol. 168, pp. 5629- 5637 ,(2002) , 10.4049/JIMMUNOL.168.11.5629
Tianyi Wang, William P. Lafuse, Bruce S. Zwilling, NFκB and Sp1 Elements Are Necessary for Maximal Transcription of Toll-like Receptor 2 Induced by Mycobacterium avium Journal of Immunology. ,vol. 167, pp. 6924- 6932 ,(2001) , 10.4049/JIMMUNOL.167.12.6924
Wei Li, Carmella D. Whaley, Jody L. Bonnevier, Anna Mondino, Molly E. Martin, Kjersti M. Aagaard-Tillery, Daniel L. Mueller, CD28 signaling augments Elk-1-dependent transcription at the c-fos gene during antigen stimulation. Journal of Immunology. ,vol. 167, pp. 827- 835 ,(2001) , 10.4049/JIMMUNOL.167.2.827
Toshibumi Shimokawa, Chisei Ra, C/EBPα and Ets Protein Family Members Regulate the Human Myeloid IgA Fc Receptor (FcαR,CD89) Promoter The Journal of Immunology. ,vol. 170, pp. 2564- 2572 ,(2003) , 10.4049/JIMMUNOL.170.5.2564
Hiroaki Hemmi, Tsuneyasu Kaisho, Kiyoshi Takeda, Shizuo Akira, The Roles of Toll-Like Receptor 9, MyD88, and DNA-Dependent Protein Kinase Catalytic Subunit in the Effects of Two Distinct CpG DNAs on Dendritic Cell Subsets The Journal of Immunology. ,vol. 170, pp. 3059- 3064 ,(2003) , 10.4049/JIMMUNOL.170.6.3059