MicroPET Outperforms Beta-Microprobes in Determining Neuroreceptor Availability under Pharmacological Restriction for Cold Mass Occupancy.

摘要: Both non-invasive micro-positron emission tomography (μPET) and in situ beta-microprobes have the ability to determine radiotracer kinetics neuroreceptor availability vivo. Beta-microprobes were proposed as a cost-effective alternative μPET, but literature revealed conflicting results most likely due methodological differences inflicted tissue damage. The current study has three main objectives: (i) evaluate theoretical advantages of beta-microprobes; (ii) perform μPET imaging assess impact (beta-micro)probe implantation on relative tracer delivery (R1) receptor occupancy (nondisplaceable binding potential, BPND) rat brain; (iii) investigate whether beta-microprobe recordings produce robust when pharmacological restriction for cold mass dose (tracer condition) is imposed. We performed acquisitions (n=61) naive animals, dummy probe implanted animals (outer diameter: 0.75 1.00 mm) using two different radiotracers with high affinity striatum: [11C]raclopride (n=29) [11C]ABP688 (n=32). In addition, completed or without an imposed occupancy. estimated BPND R1 values simplified reference method (SRTM). µPET (0.75 mm: -13.01±0.94%; -13.89±1.20%) -29.67±4.94%; -39.07±3.17%) significantly decreased at implant side versus contralateral intact side. A similar comparison µPET, demonstrated (p<0.05) (-19.09±2.45%) (-38.12±6.58%) striatum mm implant, not implant. Particularly conditions, despite lower partial volume effects, proved unfit representative destruction associated insertion. advise use obtain accurate concerning delivery, keeping mind effects which it possible correct.