Random Amplification of Polymorphic DNA for Tracing and Molecular Epidemiology of Listeria Contamination in a Cheese Plant.

摘要: The objective of this study was to evaluate a modified random amplification polymorphic DNA (RAPD) technique as tool determine routes Listeria contamination in dairy production facilities. Fifty-two strains L. monocytogenes and innocua were isolated from an Austrian cheese factory, the isolates analyzed by RAPD technique. Initially, all subjected with 20 arbitrary 10-mer oligonucleotide primers, grouped into 10 groups. In further step, 200 more primers evaluated comparing patterns several pools each subgroups. This approach established that (i) capable identifying single strain pool closely related strains, (ii) no could be identified additional primers. We also compared our differentiation previously published methods typing, found them less accurate. Forty-eight serotyped serotypes 1/2a, 1/2b, 3c 4b ; these, 41 clustered group identical fingerprints comprising both 1/2a 1/2b six four serotype isolate constituted unique group. remaining 6b, represented separate Thus, different serovars composed group, same groups, indicating possible recombinational mechanism for generations serovars. Conversely, similar might not operational innocua, since serovar typing concordant species. PCR detection hly locus encoding listeriolysin O, major virulence factor, but suggests field are potentially pathogenic.