Molecular based diagnosis of Rinderpest and Peste des petits ruminants virus in Pakistan.
作者： Umer Farooq , Q. M. Khan , T. Barrett
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摘要: Differential diagnosis of Rinderpest (RP) and Peste des petits ruminants (PPR) by host species clinical signs is not always exact; hence, laboratory confirmation necessary to establish the cause disease distinguish these two viruses, where small animals are source infection for large ruminants. In this study, reverse transcription polymerase chain reaction (RTPCR) was standardized against RP PPR. RNA viruses isolated using denaturing solution (Solution D) containing guanidium thiocynate, sarcosyl, sodium citrate s2 mercapto-ethanol. Regular PCR morbilli specific oligonuclotides based on sequences conserved region F P-gene. These P gene primers amplified 429bp 372bp, respectively both Nested performed differentiate PPR F-gene amplicons F3A/F4A F1A/F2A primers, respectively. set a product 235bp 309bp PPR, The results study indicated that RT-PCR can be successfully used detection differential
cabdirect.org参考文章(32)
T. Barrett, Rinderpest and Distemper Viruses Encyclopedia of Virology (Third Edition). pp. 497- 507 ,(2008) , 10.1016/B978-012374410-4.00496-9
D Bhavasar, RA Venkatesan, Shaila, K Venugopal, Purushothaman, Peste des petits ruminants of sheep in India. Veterinary Record. ,vol. 125, pp. 602- 602 ,(1989) , 10.1136/VR.125.24.602-A
P. L. Roeder, G. Abraham, G. Kenfe, T. Barrett, Peste des petits ruminants in Ethiopian goats Tropical Animal Health and Production. ,vol. 26, pp. 69- 73 ,(1994) , 10.1007/BF02239901
W. P. Taylor, T. Barrett, Rinderpest and Peste Des Petits Ruminants Diseases of Sheep, Fourth Edition. pp. 460- 469 ,(2008) , 10.1002/9780470753316.CH61
M.A. Forsyth, T. Barrett, Evaluation of polymerase chain reaction for the detection and characterisation of rinderpest and peste des petits ruminants viruses for epidemiological studies. Virus Research. ,vol. 39, pp. 151- 163 ,(1995) , 10.1016/0168-1702(95)00076-3
E.C. Anderson, A. Hassan, J. Anderson, Observations on the pathogenicity for sheep and goats and the transmissibility of the strain of virus isolated during the rinderpest outbreak in Sri Lanka in 1987 Veterinary Microbiology. ,vol. 21, pp. 309- 318 ,(1990) , 10.1016/0378-1135(90)90002-D
Adama Diallo, Thomas Barrett, Monique Barbron, Shaila M. Subbarao, William P. Taylor, Differentiation of rinderpest and peste des petits ruminants viruses using specific cDNA clones. Journal of Virological Methods. ,vol. 23, pp. 127- 136 ,(1989) , 10.1016/0166-0934(89)90126-2
K. Pandey, M. Baron, T. Barrett, Differential diagnosis of rinderpest and PPR using biotinylated cDNA probes. Veterinary Record. ,vol. 131, pp. 199- 200 ,(1992) , 10.1136/VR.131.9.199
T. Barrett, I.K.G. Visser, L. Mamaev, L. Goatley, M.-F. van Bressem, A.D.M.E. Osterhaus, Dolphin and porpoise morbilliviruses are genetically distinct from phocine distemper virus. Virology. ,vol. 193, pp. 1010- 1012 ,(1993) , 10.1006/VIRO.1993.1217
G. Meyer, A. Diallo, The nucleotide sequence of the fusion protein gene of the peste des petits ruminants virus: the long untranslated region in the 5'-end of the F-protein gene of morbilliviruses seems to be specific to each virus. Virus Research. ,vol. 37, pp. 23- 35 ,(1995) , 10.1016/0168-1702(95)00013-G