Metastatic state of parent cells influences the uptake and functionality of prostate cancer cell-derived extracellular vesicles

摘要: Extracellular vesicles (EVs), including microvesicles and exosomes, mediate intercellular signalling which has a profound role in cancer progression the development of metastasis. Internalisation EVs can prompt functional changes recipient cells, nature depends on molecular composition cargo EVs. We hypothesised that metastatic stage cancerous parent cells would determine uptake efficacy subsequent effects respective cell-derived To address this question, we compared internalisation derived from two site-derived prostate cell lines (PC-3 LNCaP), human telomerase reverse transcriptase immortalised primary malignant epithelial (RC92a/hTERT), benign line (PNT2). isolated PC-3 LNCaP were more efficiently internalised by PNT2 to RC92a/hTERT or as determined high content microscopy, confocal flow cytometry. EV was also influenced phase cycle, so an increased EV-derived fluorescence signal observed at G2/M G0/G1 S phases. Finally, differences functions based source. Proliferation lesser extent enhanced particularly metastatic-site-derived comparison whereas migration all RESPONSIBLE EDITOR Takahiro Ochiya, National Cancer Center, Japan.