ALG-2 directly binds Sec31A and localizes at endoplasmic reticulum exit sites in a Ca2+-dependent manner.
作者： Hideki Shibata , Hironori Suzuki , Haruna Yoshida , Masatoshi Maki
DOI: 10.1016/J.BBRC.2006.12.101
关键词:
摘要: Intracellular localization of the penta-EF-hand Ca2+-binding protein ALG-2 in HeLa cells was investigated by immunofluorescent confocal microscopy using a polyclonal antibody. In addition to its presence nucleus, found be distributed punctate pattern cytoplasm, where it partly co-stained with an endoplasmic reticulum (ER) exit site marker p125. vitro GST pull down analysis demonstrated that and alternatively spliced isoform interact COPII component Sec31A Ca2+-dependent manner, biotin-labeled overlay assay revealed direct binding Sec31A. Biochemical microscopic analyses showed enriched at Sec31A-localizing membrane compartments upon stimulation Ca2+ ionophore A23187. contrast, treatment membrane-permeant chelator BAPTA-AM led dispersion throughout significant loss perinuclear region. These findings establish as novel target for provide framework studies on roles ER-Golgi transport.
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sci-hub.se 参考文章(21)
Yasuyuki Kitaura, Shinji Matsumoto, Hirokazu Satoh, Kiyotaka Hitomi, Masatoshi Maki, Peflin and ALG-2, Members of the Penta-EF-Hand Protein Family, Form a Heterodimer That Dissociates in a Ca2+-dependent Manner Journal of Biological Chemistry. ,vol. 276, pp. 14053- 14058 ,(2001) , 10.1074/JBC.M008649200
Kevin W.-H. Lo, Qiang Zhang, Ming Li, Mingjie Zhang, Apoptosis-linked gene product ALG-2 is a new member of the calpain small subunit subfamily of Ca2+-binding proteins. Biochemistry. ,vol. 38, pp. 7498- 7508 ,(1999) , 10.1021/BI990034N
Kohei Arasaki, May Taniguchi, Katsuko Tani, Mitsuo Tagaya, RINT-1 Regulates the Localization and Entry of ZW10 to the Syntaxin 18 Complex Molecular Biology of the Cell. ,vol. 17, pp. 2780- 2788 ,(2006) , 10.1091/MBC.E05-10-0973
Keiichi Katoh, Hidenori Suzuki, Yoshinori Terasawa, Takako Mizuno, Jiro Yasuda, Hideki Shibata, Masatoshi Maki, The penta-EF-hand protein ALG-2 interacts directly with the ESCRT-I component TSG101, and Ca2+-dependently co-localizes to aberrant endosomes with dominant-negative AAA ATPase SKD1/Vps4B. Biochemical Journal. ,vol. 391, pp. 677- 685 ,(2005) , 10.1042/BJ20050398
Hirokazu Satoh, Yoshimi Nakano, Hideki Shibata, Masatoshi Maki, The penta-EF-hand domain of ALG-2 interacts with amino-terminal domains of both annexin VII and annexin XI in a Ca2+-dependent manner. Biochimica et Biophysica Acta. ,vol. 1600, pp. 61- 67 ,(2002) , 10.1016/S1570-9639(02)00445-4
Masatoshi Maki, Yasuyuki Kitaura, Hirokazu Satoh, Susumu Ohkouchi, Hideki Shibata, Structures, functions and molecular evolution of the penta-EF-hand Ca2+-binding proteins. Biochimica et Biophysica Acta. ,vol. 1600, pp. 51- 60 ,(2002) , 10.1016/S1570-9639(02)00444-2
Rémy Sadoul, Do Alix and ALG-2 really control endosomes for better or for worse? Biology of the Cell. ,vol. 98, pp. 69- 77 ,(2006) , 10.1042/BC20050007
Thomas Slagsvold, Krupa Pattni, Lene Malerød, Harald Stenmark, Endosomal and non-endosomal functions of ESCRT proteins Trends in Cell Biology. ,vol. 16, pp. 317- 326 ,(2006) , 10.1016/J.TCB.2006.04.004
Keiichi Katoh, Hideki Shibata, Hidenori Suzuki, Atsuki Nara, Kazumi Ishidoh, Eiki Kominami, Tamotsu Yoshimori, Masatoshi Maki, The ALG-2-interacting Protein Alix Associates with CHMP4b, a Human Homologue of Yeast Snf7 That Is Involved in Multivesicular Body Sorting Journal of Biological Chemistry. ,vol. 278, pp. 39104- 39113 ,(2003) , 10.1074/JBC.M301604200
Wakako Shimoi, Ichiko Ezawa, Koji Nakamoto, Shihoko Uesaki, Gavin Gabreski, Meir Aridor, Akitsugu Yamamoto, Masami Nagahama, Mitsuo Tagaya, Katsuko Tani, p125 Is Localized in Endoplasmic Reticulum Exit Sites and Involved in Their Organization Journal of Biological Chemistry. ,vol. 280, pp. 10141- 10148 ,(2005) , 10.1074/JBC.M409673200