Reference Values for Urinary Steroids in Japanese Newborn Infants: Gas Chromatography/Mass Spectrometry in Selected Ion Monitoring

摘要: Urinary steroid profile analysis using gas chromatography/mass spectrometry (GC/MS) has been reported for the diagnosis of abnormal steroidogenesis in newborn infants with some success. We tried to establish reference values 63 urinary steroids Japanese infant, GC/MS selected ion monitoring (SIM) that utilizes two characteristic mass ions each definitive identification. studied 36 healthy full-term (1-56 days age) on spot urine samples define (mg/g creatinine, median and 10-90 percentile range) investigate possible difference between daytime nighttime levels. also 23 adult females (20-24 years 24-hour-urine comparison infants. Fifty metabolites DHEA, pregnenolone, 17-hydroxypregnenolone, androstenedione, progesterone, 17-hydroxyprogesterone, 21-deoxycortisone, corticosterone, 18-hydroxycorticosterone, aldosterone, 18-hydroxycortisol, 11-deoxycortisol, cortisone, cortisol, estrogen infant were measurable without interference, but 13 11-hydroxyandrostenedione, 11-deoxycorticosterone, 11-dehydrocorticosterone, 21-deoxycortisol, 11-deoxycortisol cortisol unmeasurable due interference fetal cortex as confirmed by peak area ratios ions. All control interference. 16α-, 16β-, 15β-hydroxy 3β-hydroxy-5-en-steroids, 6β-, 18-hydroxy 11-oxo-metabolites corticosteroids significantly higher than those adults previously reported. showed little circadian variation infants, indicating can substitute 24-hour urine.