RNA Sequencing Analysis of the Gametophyte Transcriptome from the Liverwort, Marchantia polymorpha

摘要: The liverwort Marchantia polymorpha is a member of the most basal lineage land plants (embryophytes) and likely retains many ancestral morphological, physiological molecular characteristics. Despite its phylogenetic importance availability previous EST studies, M. polymorpha’s lack economic limits accessible genomic resources for this species. We employed Illumina RNA-Seq technology to sequence gametophyte transcriptome polymorpha. cDNA libraries from 6 different male female developmental tissues were sequenced delineate global view transcriptome. Approximately 80 million short reads obtained assembled into non-redundant set 46,533 transcripts (> = 200 bp) 46,070 loci. average length N50 757 bp 471 bp, respectively. Sequence comparison with proteins embryophytes resulted in annotation 43% transcripts. also compared expressed tags (ESTs), approximately 69.5% appeared be novel. Twenty-one percent assigned GO terms improve annotation. In addition, 6,112 simple repeats (SSRs) identified as potential markers, which may useful studies genetic diversity. A comparative genomics approach revealed that substantial proportion genes (35.5%) conserved across phylogenetically related species, such Selaginella Physcomitrella, 580 are potentially unique liverworts. Our study presents an extensive amount novel information This will serve valuable resource further molecular, evolutionary well isolation characterization functional involved sex differentiation sexual reproduction liverwort.