The mitochondrial AAA protease FTSH3 regulates Complex I abundance by promoting its disassembly.

摘要: ATP is generated in mitochondria by oxidative phosphorylation. Complex I (NADH:ubiquinone oxidoreductase or NADH dehydrogenase) the first multisubunit protein complex of this pathway, oxidising and transferring electrons to ubiquinone pool. Typically mutants display a slow growth rate compared wild-type plants. Here, using forward genetic screen approach for restored mutant, we have identified mitochondrial dependent metalloprotease, Filamentous Temperature Sensitive H 3 (FTSH3), as factor that required disassembly I. An ethyl methanesulfonate-induced mutation FTSH3, named rmb1 (restoration biogenesis 1), abundance plant growth. Complementation could be achieved with FTSH3 lacking proteolytic activity, suggesting unfoldase function has role disassembly. The introduction an additional, independent, extensively characterised ndufs4, resulted similar increases partial restoration These results show degradation plays determining its steady-state thus turnover may vary under different conditions.