Quantitative differences in the cyclic AMP-lipolysis relationships for isoproterenol and forskolin.

摘要: The relationships between cyclic AMP (cAMP) levels and glycerol release (lipolysis) were determined for isoproterenol forskolin under varied conditions in the isolated fat cell of rat. Prevention inhibitory action endogenous adenosine [by deaminase (100 mU/ml) or theophylline (3.3 X 10(-4) M)] resulted increased cAMP rates lipolysis with forskolin. However, relationship remained same all conditions. N6-phenylisopropyladenosine (PIA; an analog adenosine) abolished increase level produced by (10(-7) M) (10(-6) lipolytic response to PIA failed inhibit completely isoproterenol. Dose-response curves presence absence deaminase. inhibited both also responses that associated increases levels, i.e., high concentrations alone A plot against corresponding agreed previous observations differs from At any concentration was greater than possibility a cAMP-independent discussed.