Citron, a Rho-Target, Interacts with PSD-95/SAP-90 at Glutamatergic Synapses in the Thalamus

摘要: Proteins of the membrane-associated guanylate kinase family play an important role in anchoring and clustering neurotransmitter receptors postsynaptic density (PSD) at many central synapses. However, relatively little is known about how these multifunctional scaffold proteins might provide a privileged site for activity- cell type-dependent specification signaling machinery. Rho pathway has classically been implicated mechanisms axonal outgrowth, dendrogenesis, migration during neural development, but its contribution remains unclear synapses mature CNS. Here, we present evidence that Citron, Rho-effector brain, enriched PSD fraction interacts with PSD-95/synapse-associated protein (SAP)-90 both vivo vitro . Citron colocalization PSD-95 occurred, not exclusively certainly, glutamatergic limited set neurons, such as thalamic excitatory neurons; expression, however, could be detected principal neurons hippocampus cerebellum adult mouse brain. In heterologous system, was shown to form heteromeric complex only also NMDA receptors. Thus, Citron–PSD-95/SAP-90 interaction may region- type-specific link between cascade synaptic receptor complex.