Herpesvirus-based infectious titering of recombinant adeno-associated viral vectors

摘要: Studies in animals and human clinical trials demonstrate the safety persistence of recombinant adeno-associated viral (rAAV) serotype 2 vectors a variety tissues. rAAV other serotypes are also being developed for efficient gene transfer. To date, literature describing these has relied on physical or transducing titers to determine dose, but few, if any, infectious have been presented. This is due large part lack reagents methods that would facilitate titering than 2. Here, we describe AAV2 ITR-containing pseudotyped with AAV capsid their utility by rAAV1 rAAV5 vectors. Cell lines screened optimal transduction using vector particular expresses marker transgene. Once cell line matched, herpes simplex virus expressing rep cap genes provides helper functions amplify genome. The genomes then detected titer calculated. These generate reliable different serotypes, thus enhancing product characterization reducing risk future applications.