Relationship betweenN-Methyl-D-aspartate Receptor NR1 Splice Variants and NR2 Subunits

摘要: The N-methyl-D-aspartate (NMDA) subtype of ionotropic glutamate receptor is assembled from one NR1 subunit, expressed in eight splice variants, and four NR2 subunits (NR2A-D). combination variants determines the pharmacological physiological properties receptor. In present study we investigated relationship between rat brain using a series antibodies selective for cassettes, which vary NR2A NR2B. Sodium deoxycholate at pH 9.0 solubilized about 35% receptor, was intact based on co-immunoprecipitation chemical cross-linking cross-linked product contained three high molecular weight components, Mr = 603,000, 700,000, 750,000, were immunolabeled with to subunits. Immunoprecipitation analyses NR2B showed no preferential assembly variants. There little NR2B, suggesting that most NMDA complexes contain only these However, can least two different developing conditions solubilization complexes, observed differential nearly insoluble Triton X-100 both microsomal synaptic membrane fractions, while readily soluble fraction but fraction. These results suggest subunit modified when it incorporated into membrane, possibly by strengthening its interaction or another protein.