Covalent immobilization of an antimicrobial peptide on poly(ethylene) film
作者： Matthew D. Steven , Joseph H. Hotchkiss
DOI: 10.1002/APP.27638
关键词:
摘要: An antimicrobial film was produced by covalently attaching synthetic peptide E14LKK to poly(ethylene) film. is a 14 residue, magainin-class with broad-spectrum activity. The surface first oxidized chromic/sulphuric acid, then PEGylated using carbodiimide chemistry attach ω-amino-α-carboxyl-poly(ethylene glycol) (PEG). coupled the free terminus of PEG, again coupling. Surface contact angles for distilled water decreased from 101° initially 61° following oxidation and 45° PEGylation. Film showed expected changes during modifications: dye adsorption assays indicated in number acidic basic groups X-ray photoemission spectroscopy increasing oxygen nitrogen levels. Antimicrobial activity demonstrated broth cultures against E.coli: growth reduced atleast 3 log cycles compared controls. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci,
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