Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice.

摘要: The Zika virus (ZIKV) can induce inflammation in immunoprivileged organs (e.g., the brain and testis), leading to Guillain-Barre syndrome damaging testes. During an infection with ZIKV, immune cells have been shown infiltrate into tissues. However, cellular mechanisms that define protection and/or immunopathogenesis of these during a ZIKV are still largely unknown. Herein, we describe methods evaluate virus-specific T-cell functionality ZIKV-infected mice. These include a) vaccine inoculation Ifnar1-/- mice; b) histopathology, immunofluorescence, immunohistochemistry assays detect brain, testes, spleen; c) preparation tetramer ZIKV-derived epitopes; d) detection ZIKV-specific T monocytes isolated from spleen. Using approaches, it is possible antigen-specific infiltrated functions infection: potential via clearance exacerbate inflammation. findings may also help clarify contribution induced by immunization against ZIKV.