Effects of low temperature, genotype and culture media on in vitro androgenic answer of pepper ( Capsicum annuum L.)

摘要: Anther culture is one of the most important and useful tool to create pure lines for plant breeding programs rapidly. Some pepper genotypes are recalcitrant embryogenic frequency in anther still low or reaction not observed at all. Temperature stress (low high) can facilitate switching microspore sporophyte developmental pathway. In this study, some differences were found among media variants, depending on duration cold treatment flower buds. Experimental results indicated that efficiency decreased under low-temperature stress. Nevertheless, positive effect pretreatment direct embryo induction was obtained four genotypes—cultivar Hebar, hybrid 50/01 668/02 1312/02. Increasing after variants C (24 h) MS-3 + (24 48 h), while medium variant C-0 formation registered only 48 h pretreatment. These show donor have specific requirements type temperature also androgenesis with higher frequency.