Response of soil microbial communities to contrasted histories of phosphorus fertilisation in pastures

摘要: Abstract Several microbial taxa that potentially affect soil phosphorus (P) biogeochemical cycling and plant nutrition have been identified, in particular some members of the Actinobacteria, Pseudomonas , arbuscular mycorrhizal fungi (AMF) found to solubilise P enhance uptake. This study investigated diversity selected key within a pastoral agricultural system, address hypothesis if these an intrinsic association P, long-term P-manipulation should present selective pressure. We defined this pressure as shift community assemblage, based on either PCR-DGGE banding patterns or taxon-specific quantitative polymerase chain reaction (qPCR) (mycorrhizae). The work was conducted fertiliser field trial site where status had altered for ∼40 years, with total ranging from 464 1102 mg kg −1 soil. structure both Actinobacteria communities differ according treatment ( were associated underlying fertility gradient (soil C:P ratio; ρ  = 0.481;  = 0.019); no significant links between physicochemical properties found. As general bacterial α-Proteobacterial did not vary treatments  > 0.05), are intrinsically linked is supported. Similarly, strongly influenced by  = 0.002), particularly strong differences control (no fertiliser) fertilised plots, meaning addition per se more important than quantity. P-fertilizer application increased AMF. AMF plot dominated Glomus intraradices claroideum ; may highest fitness under low conditions. fungal structure, assessed denaturing gel electrophoresis (DGGE), also varied over  = 0.001), however variation correlated (biota and/or environment matching; BIO-ENV test). Our results expand our knowledge microbiology supporting pasture soils. Furthermore, we demonstrate AMF, proposed be involved supplying plants, status.