Flexibility of an Antibody Binding Site Measured with Photon Echo Spectroscopy

作者: Ralph Jimenez , David A. Case , Floyd E. Romesberg

DOI: 10.1021/JP013110G

关键词:

摘要: The lock-and-key and induced fit models of antigen binding by antibodies are topics considerable importance to immunology protein biochemistry in general. These ascribe different degrees flexibility the antibody combining site may therefore be distinguished measuring response force exerted charge redistribution structural reorganization an optically excited antigen. Spectroscopic characterization magnitude time scale this is a basis for comparing antibodies. This approach used study panel elicited chromophoric 8-methoxypyrene-1,3,6,trisulfonic acid. energies chromophore-antibody complexes determined found distributed over wide range. Three pulse photon echo peak shift spectroscopy measure scales process one complex. motions occur on ranging from 75 femtoseconds 67 picoseconds. Structural origins observed discussed.

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