Quantification of Methanosaeta Species in Anaerobic Bioreactors Using Genus- and Species-Specific Hybridization Probes

摘要: A BSTRACTTo evaluate the role of Methanosaeta spp. in a variety anaerobic environments, small-subunit rRNA targeted oligonucleotide hybridization probes were developed and experimentally characterized. The designed to be genus specific for species concilii thermophila. temperature dissociation was determined each probe. Probe specificities using diverse collection Archaea through an evaluation probe nesting samples from bioreactors. Cell fixation conditions fluorescence situ hybridizations also evaluated. Although permeability methanogens variable, M. cells could permeabilized range paraformaldehyde ethanol based conditions. Using newly together with previously methanogens, it that dominant aceticlastic bioreactors when acetate concentrations low. Their levels higher granular sludge than those flocculent sludge. In lab-scale upflow blanket reactors, as high 30% total rRNA.