Amplicon mapping and transcriptional analysis pinpoint cyclin L as a candidate oncogene in head and neck cancer.

摘要: DNA gains targeting the 3q chromosome are common in head and neck squamous cell carcinomas, as well lung, ovarian, cervical cancer. Several candidate oncogenes located on were proposed, i.e., PIK3CA, p63, eIF-5A2. However, none of these genes was found included a narrow high-level amplification. Recently, microarray-based comparative genomic hybridization (array CGH) developed for high-resolution screening deletions amplifications tumor genomes. In this study, by hybridization, we 3q25.3 amplification cancer line. We precisely delimited 3-Mb length-amplified segment semiquantitative PCR measured transcriptional level every gene (RefSeq full-length mRNA) inside cDNA microarray quantitative reverse transcription-PCR. Four overexpressed three lines with increased copy number, compared control tongue extended analysis four to 20 carcinomas. Only one gene, cyclin L (ania-6a), is commonly primary tumors corresponding normal tissues. This previously pinpointed role promoting cycle entry. Thus, propose oncogene