Interaction of gold nanoparticles with protein: A spectroscopic study to monitor protein conformational changes
作者： Nishima Wangoo , C. Raman Suri , G. Shekhawat
DOI: 10.1063/1.2902302
关键词:
摘要: Gold nanoparticles (GNPs) conjugated with biomolecules are promising building blocks for assembly into nanostructured functional materials developing biomarker platforms because of their size dependent optical and electrical properties. Biocompatible GNPs were synthesized using glutamic acid as a reducing agent the interaction between bovine serum albumin (BSA) was investigated fluorescence circular dichroism (CD) spectroscopies. The binding constant (Kb) protein to determined by measuring quenching intensity tryptophan residues molecules after conjugation. conformational change in BSA at its native form conjugation confirmed that undergoes more flexible state on boundary surface bioconjugation. CD studies further showed decrease α-helical content results conformation larger higher conce...
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