Acute stimulation of steroidogenesis in corpus luteum and adrenal cortex by peptide hormones. Rapid induction of a similar protein in both tissues.

摘要: Two-dimensional electrophoresis was used to detect a protein (ic) synthesized in rat corpus luteum cells response acute stimulation by human chorionic gonadotropin or dibutyryl cyclic AMP. This induced ic is isoelectric at pH 6.5 (isoelectric focusing) and has an apparent molecular weight of 28,000 (sodium dodecyl sulfate electrophoresis). The AMP dose time course synthesis the parallel those progesterone stimulated luteal cells. Additionally, cycloheximide, which inhibits increase formation caused cAMP, also ic. Proteolytic polypeptide mapping suggests that very similar primary structure another (pc), same as ic, differs from pI, only unstimulated These maps demonstrate close similarity pc two proteins p i, control adrenocorticotropic hormone-stimulated adrenal cortex cells, respectively (Krueger, R. J. Orme-Johnson, N. (1983) Biol. Chem. 258, 10159-10167). In both luteum, binding tissue-specific hormone acts via cAMP cause increased steroidogenesis induction i (ic), with dependence. Also tissues, inhibition level translation (e.g. cycloheximide addition) causes steroid production. correlation between different tissues conditions these may be common intermediaries hormones endocrine tissues.