BAI3, CDX2 and VIL1: a panel of three antibodies to distinguish small cell from large cell neuroendocrine lung carcinomas
作者： Muhammad F Bari , Helen Brown , Andrew G Nicholson , Keith M Kerr , John R Gosney
DOI: 10.1111/HIS.12278
关键词:
摘要: Aims Discriminating small-cell lung carcinoma (SCLC) from large-cell neuroendocrine (LCNEC) rests on morphological criteria, and reproducibility has been shown to be poor. We aimed identify immunohistochemical markers assist this diagnosis. Methods results Gene expression profiling laser captured frozen tumour samples eight SCLC LCNEC tumours identified a total of 888 differentially expressed genes (DEGs), 23 which were validated by qRT–PCR. Antibodies four selected gene products then evaluated as cohort 173 formalin-fixed paraffin-embedded (FFPE) SCLC/LCNEC samples, including 26 indeterminate without consensus diagnosis. Three markers, CDX2, VIL1 BAI3, gave significantly different results in the two types (P < 0.0001): CDX2 combination (either marker positive) showed sensitivity specificity 81% for while BAI3 89% 75% SCLC. Of 15 (58%) an immunophenotype suggesting either or LCNEC, (31%) staining both types, three (11%) negative all markers. Conclusion A panel VIL1, is useful adjunct diagnosis these types.
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