Rapid separation of lipid classes in high yield and purity using bonded phase columns.

摘要: A method utilizing aminopropyl bonded phase (Bond Elut) columns has been developed to separate lipid mixtures into individual classes in high yield and purity. Up ten can be processed 1 hr the are reusable after suitable washing. Although was with standard mixtures, it shown that is also applicable biological extracts. Due rapidity yields (greater than 95%) of this procedure, superior preparative HPLC or TLC, other chromatographic methods for separation subsequent analysis.