Molecule-specific imaging with mass spectrometry and a buckminsterfullerene probe: application to characterizing solid-phase synthesized combinatorial libraries.

摘要: We employ a newly developed buckminsterfullerene (C60) primary ion beam with time-of-flight secondary mass spectrometry to create molecule-specific images of resin particles employed in the solid-phase synthesis peptide combinatorial libraries. This new cluster source, when operated at an incident energy 20 keV, is remarkably effective desorbing small peptides directly from polymer surface and opens possibilities for characterizing large arrays diverse sets molecules. In addition, C60 may be focused spot 1.5 μm diameter, enabling single 100 acquired. report three significant aspects associated utilizing projectile that show how this technology can taken more advanced level, especially compared results obtained conventional atomic ions. First, useful yield molecular ions generally observed enhanced by least 3 orders magnitude over ...