Novel human, mouse and xenopus genes encoding a member of the RAS superfamily of low-molecular-weight GTP-binding proteins and its downregulation in W/WV mouse jejunum.

摘要: Background and Aim:  Interstitial cells of Cajal (ICC) are pacemakers mediators neurotransmission in gastroenteric smooth muscles. require cellular signaling via KIT, a receptor tyrosine kinase, for development maintenance phenotype. Much the evidence demonstrating functions ICC comes from studies W/W V mutant mice, which have reduced KIT function. The aim present study was to differentially examine gene expression small intestines wild-type mice. Methods:  RNA jejunum mice analyzed using differential display method. Results:  One candidate gene, encoding novel GTPase RAS superfamily, significantly suppressed both fed starved W/WV mice. full-length clone murine its human xenopus counterparts were designated GTP-binding protein, 28 kDa (G28K). Human G28K cDNA encodes protein 258 amino acids with homology cell division cycle 42/G25K protein. This is located at 1q42.11–q42.3. abundantly expressed stomach intestine. Semi-quantitative reverse transcription–polymerase chain reaction analysis revealed mRNA within single isolated ICC. Conclusions:  Gene showed that intestine G28K. specific downregulation high intestinal tissue suggest might be associated function humans.